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Edman-N sequencing

Edman-N sequencing

Region: Shanghai
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  • Contract Manufacturing: CRO
  • Main Sales Markets: Asia
  • Sample Provided: no

Protein biosynthesis starting from the N-terminal, the amino acid sequence of N-terminal has a strong impact on the biological functions, such as the half-life of protein, the sub cellular localization of protein. Certain posttranslational modifications involving the protein function and the stability also occur on N-terminal. Therefore by analyzing the N-terminal sequence, the person can analyze the spatial structures of proteins and evaluate the biological function of proteins.

With the development of modern pharmaceutical industry, there have been a lot of protein and polypeptide drugs molecules. Analyzing the N-terminal amino acid sequence is a critical link in pharmaceutical quality control.

At present, there are two main methods to study the protein n-terminal sequence -non-mass spectrum technology, such as traditional Edman degradation, RT-PCR reverse transcription, and mass spectrometry. Each method has its own advantages and shortcomings. The technology based on the traditional Edman degradation has become more common on the market.

Utilizing the automatic protein sequenator (PPSQ , Shimadzu) analysis, unambiguous data of protein N-terminal amino acid sequence will be delivered.

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